What is an Antibody Fragment?
Protein engineering techniques provide a means for minimizing the size of antibodies into Fab fragments, scFv or even single-domain antibody fragments such as VH, VL or VHH. These genetically engineered antibody fragments provide new opportunities for therapeutic and diagnostic applications. However, unlike full-chain monoclonal antibodies where Protein A based affinity adsorbents provide a convenient platform for capture and purification, antibody fragments which lack an Fc region frequently require purification by using a combination of non-affinity-based chromatography methods.
What is the function of an Antibody Fragment?
F(ab) and F(ab’)2 fragment antibodies eliminate non-specific binding between Fc portions of antibodies and Fc receptors on cells (such as macrophages, dendritic cells, neutrophils, NK cells and B cells). They can penetrate tissues more efficiently due to their smaller size and they do not interfere with anti-Fc mediated antibody detection.
Fabsorbent™ F1P HF
Prometic Bioseparations has developed an affinity adsorbent for the capture and purification of a wide range of antibody fragments. Fabsorbent™ F1P HF is a synthetic-ligand light-chain binding adsorbent which can be used as a superior alternative to Protein L for the capture and purification of antibody fragments including monovalent antibody fragments (e.g. Fab, scFv), engineered antibody variants and single domain antibodies.
Key Features of Fabsorbent™ F1P HF:
- Fab’ expressed in E.coli
- Mammalian Fab fragments from papain catalysed antibody digests
- Mammalian F(ab’)2 fragments from pepsin catalysed antibody digests
- Human antibody λ and κ light chains
- ScFv expressed in E.coli
- Single domain antibody fragments expressed in E. coli
- Human, bovine, ovine and murine antibodies and antibody fragments