Antibodies (Polyclonal and Monoclonal IgG)

What is a Polyclonal Antibody?

Polyclonal antibodies are a collection of antibodies each recognising different epitopes on the same antigen molecule. Polyclonal antibodies are easier and inexpensive to produce by immunising an animal (horse, sheep etc.) with the target antigen such as protein. Once the immunised animal has produced an immune response specific for the target antigen, polyclonal antibodies can be detected in its blood. The blood can be harvested and centrifuged to remove blood cells to provide plasma which contains a mixture of antibodies and other proteins as well as low molecular weight components. Polyclonal antibodies can be purified from plasma by affinity chromatography.

What is a Monoclonal Antibody?

Monoclonal antibody is an immunoglobulin which is produced in the laboratory from a clone of a single parent cell and generally have a monovalent affinity for single epitope of an antigen. Several different monoclonal antibodies have been produced using cell culture technology and the produced antibodies are secreted into the cell culture medium from which they can be purified after the removal of cells by affinity chromatography.

What are Antibodies used for?

Due to their high specificities and selectivity’s, both polyclonal and monoclonal antibodies are used in research, diagnostic and therapeutic applications. In research, they are routinely used to identify and quantify molecules in procedures such as Western Blots, Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry and Flow Cytometry. Antibodies are the main component of many diagnostic assays for allergens, hormones, infectious agents and other biological blood markers of a disease state. Purified human polyclonal antibodies obtained from plasma (ivIgG) are used for the treatment of immune deficiencies. Manufactured therapeutic monoclonal antibodies are widely used to treat several types of diseases including cancer, rheumatoid arthritis, psoriasis and multiple sclerosis.

MAbsorbents®

MAbsorbents® were developed to mimic the Phe-132, Tyr-133 dipeptide binding site in the hydrophobic core structure of Protein A. We have developed IgG binding ligand libraries using a triazine scaffold substituted with aromatic amines. The resultant synthetic bifunctional ligands exhibit high affinities for human immunoglobulin G. Hence, MAbsorbent® A2P and A2P LL provide excellent purification performance.

MAbsorbents® effectively bind a wide variety of human and mammalian polyclonal antibodies (including bovine, mouse, sheep, goat, horse and rabbit) as well as whole monoclonal antibodies, humanized antibody chimeras and antibody fragments.

Key features of MAbsorbents®:

  • Polyclonal IgG and all sub-classes
  • Monoclonal antibodies(all classes of IgG)
  • Humanized antibodies
  • Antibody fragments
  • Antibody fusion proteins

MAbsorbents®