Alkaline Phosphatase

What is Alkaline Phosphatase?

Alkaline phosphatase (orthophosphoric monoester phosphohydrolase [E.C. 3.1.3.1]) is a widely used reagent in biochemistry, molecular biology and immunodiagnostics. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols.

What is the function of Alkaline Phosphatase?

It is routinely used to dephosphorylate proteins (e.g. casein) as well as nucleic acids. Alkaline phosphatase has also been shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A[1].

Mimetic Blue® AP A6XL

Prometic Bioseparations’ Mimetic Blue® AP A6XL comprises a customised Mimetic Blue® ligand attached to beaded agarose supports, which selectively binds to a phosphonate group for the purification of alkaline phosphatase from a variety of sources, including plasma, serum and recombinant cell cultures to yield highly purified biological products.

Key features of Mimetic Blue® AP A6XL:

  • High Specificity for alkaline phosphatase
  • High dynamic binding capacity
  • Excellent flow properties
  • Robust, long-life adsorbent
  • Sanitizable with NaOH allowing multiple cycles
  • High purity, chemically defined ligand structures
  • Highly reproducible batch-to-batch manufacture to ISO9001 standards
  • Supported with comprehensive Regulatory Support Files

Mimetic Blue® AP Agarose 6XL for Purification of Alkaline Phosphatase