Serum albumin is the most abundant blood plasma protein and is produced in the liver and forms a large proportion of all plasma protein. The human version is human serum albumin, and it normally constitutes about 50% of human plasma protein.
Serum albumins are important in regulating blood volume by maintaining the oncotic pressure (also known as colloid osmotic pressure) of the blood compartment. They also serve as carriers for molecules of low water solubility this way isolating their hydrophobic nature, including lipid-soluble hormones, bile salts, unconjugated bilirubin, free fatty acids (apoprotein), calcium, ions (transferrin), and some drugs like warfarin, phenobutazone, clofibrate & phenytoin. For this reason, it is sometimes referred as a molecular “taxi”. Competition between drugs for albumin binding sites may cause drug interaction by increasing the free fraction of one of the drugs, thereby affecting potency.
Prometic Bioseparations’ range of Mimetic Blue® affinity adsorbents comprise Mimetic Blue® ligands attached to beaded agarose supports, which selectively bind to Albumin and Albumin related proteins from a variety of sources, including plasma, serum and recombinant cell cultures to yield highly purified biological products.
They are used commercially for the purification of albumin and albumin related proteins (i.e. albumin fusion proteins) but also are applicable to a variety of other proteins including adenine nucleotide binding proteins.
Key features include:
• High Specificity for albumin and albumin related proteins
• High dynamic binding capacity (>30mg/ml)
• Excellent flow properties
• Robust, long-life adsorbent
• Sanitizable with NaOH allowing multiple cycles
• High purity, chemically defined ligand structures
• Highly reproducible batch-to-batch manufacture to ISO9001 standards
• Supported with comprehensive Regulatory Support Files
• Multiple end users in biopharmaceutical manufacture
• Highly sensitive ELISA assay for leachate analysis and column verification